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? Most of the houses have electric connections. But after the spread of electricity, these Persian wheels were replaced by electric tube wells. The spread of electricity supported the Palampur farmers as it helped to change the village's irrigation system. A number of roads were closed, dozens of houses and drinking water supply schemes suffered damage and many areas were without electricity. A number of well-known artists had made their base in Andretta. Pumpkin cultivation in India is affected by severe incidence of a yellow vein mosaic disease. Land area under cultivation is practically fixed. Q. 2. Is it important to increase the area under irrigation? These seeds require a combination of chemical fertilisers and pesticides, agricultural implements like tractors, and proper irrigation facilities like electric tube wells to produce the best results. Set amongst the tea gardens in Palampur, it makes you feel like you are staying in the lap of nature. Palampur is a green hill station and a municipal council in the Kangra Valley in the Indian state of Himachal Pradesh, surrounded by tea gardens and pine forests before they merge with the Dhauladhar ranges. Set on the rising slopes of Kangra Valley before they merge with the Dhauladhar ranges.We have previously shown the association of Zucchini yellow mosaic virus (ZYMV), a potyvirus, with severe leaf curl disease of muskmelon in Pakistan. However, the ToLCPMV variant identified in association with ZYMV has a defective NSP. This is the first report of natural infection of ToLCPMV on pumpkin and association of PepLCB with yellow vein mosaic disease of pumpkin in India. We were able to identify a third begomovirus-Tomato leaf curl Palampur virus (ToLCPMV), from pumpkin showing typical symptoms of the disease at Varanasi based on the sequence of complete DNA-A genome of the virus. The complete DNA-A sequence of the virus shared more than 99% sequence identity with other ToLCPMV isolates available in the GenBank? and clustered with them in the phylogenetic analysis. Analysis of the complete nucleotide sequence of the DNA A and DNA B components of the begomovirus showed that it has the highest DNA sequence identity with ToLCPMV. Tomato leaf curl https://palampurpanorama.com virus (ToLCPMV) is a bipartite begomovirus which has been reported from India and Iran but infectious clones have not been obtained. Agrobacterium-mediated inoculation of Nicotiana benthamiana with the ToLCPMV clones obtained here did not result in symptoms.This suggested that the lack of infectivity of the ToLCPMV clones was due to the defect in DNA B. The DNA B of ToLCPMV was able to move systemically when inoculated with DNA A of the either virus. The virus appeared to be monopartite. 325 members11. Members of the genus are ssDNA viruses; the genetic material is encapsulated in twinned icosahedral particles, may be organized monopartite or bipartite material (on the basis of genomic DNA circles) and replicates via rolling circle replication (RCA)12. These ORFs encode the coat protein (AV1), precoat protein (AV2), replication initiator protein (AC1), transcription activator protein (AC2), replication enhancer protein (AC3), AC4 protein, nuclear shuttle protein (BV1) and movement protein (BC1). DNA-A possesses six open reading frames (ORFs): AV1 and AV2 are present on the virion sense strand, and AC1, AC2, AC3 and AC4 are present on the virion antisense strand. The DNA-B component comprises the BV1 and BC1 ORFs on the virion sense and antisense strands, respectively. However, inoculation of plants with the DNA A component of ToLCPMV and the DNA B component of Tomato leaf curl New Delhi virus (ToLCNDV) lead to systemic infection with leaf curl symptoms.